A Label-Free Fluorescent Assay for the Rapid and Sensitive Detection of Adenosine Deaminase Activity and Inhibition
نویسندگان
چکیده
منابع مشابه
A gold nanoparticle-based label free colorimetric aptasensor for adenosine deaminase detection and inhibition assay.
A novel strategy for the fabrication of a colorimetric aptasensor using label free gold nanoparticles (AuNPs) is proposed in this work, and the strategy has been employed for the assay of adenosine deaminase (ADA) activity. The aptasensor consists of adenosine (AD) aptamer, AD and AuNPs. The design of the biosensor takes advantage of the special optical properties of AuNPs and the interaction b...
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Based on enzyme-regulated unmodified gold nanoparticle aggregation, a visual and homogeneous assay of adenosine deaminase (ADA) activity without any other coupling enzymes, aptamers or additional modifications has been developed. The present strategy is simple, cost-effective, high throughput, selective and sensitive for ADA with a detection limit of 0.8227 U L(-1).
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ژورنال
عنوان ژورنال: Sensors
سال: 2018
ISSN: 1424-8220
DOI: 10.3390/s18082441